Coding
N4 gp8

Part:BBa_M36548:Design

Designed by: Rashmi Sharma, Bryce Bajar, Kendrick Wang   Group: Stanford BIOE44 - S11   (2011-12-07)

DNA Polymerase Inhibitor (via N4 coliphage gp8)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We derived this sequence from coliphage N4 gp8. Since N4 infects E. coli, no codon optimization for E. coli is required.

Since this function inhibits DNA replication, we advise using a medium- to low- strength RBS to prevent leaky expression during construction process.

Source

Coliphage N4 gp8

References

Yano, S.T. and L.B. Rothman-Denes (2011), A phage-encoded inhibitor of Escherichia coli DNA replication targets the DNA polymerase clamp loader. Molecular Microbiology, 79: 1325-1338.